Dr.sc. Igor Matić, dipl.ing.mol.biol.
Born 5th June 1981 in Rijeka, Croatia
Nationality: Croatian
Residency: Croatia
EDUCATION
2006-2013 PhD in Biology, University of Zagreb, Faculty of Science, Croatia
2000-2006 MSc in Molecular Biology, University of Zagreb, Faculty of Science, Croatia
1996-2000 Grammar school, Prva Rijecka Hrvatska Gimnazija, Rijeka, Croatia
EXPERIENCE
2016 – Clinical embryologist, Poliklinika IVF, Zagreb, Croatia
2013-2016 Postdoctoral Fellow, Senior Assistant, Faculty of Science, University of Zagreb, Croatia
Research in bone biology; Scientific projects titles: “Posterior cruciate ligament reconstruction: Optimization of BMP-2 gene therapy ex vivo” and “Changes in the expression of stem cell markers (Oct4, Nanog, Sox) during osteogenesis (from hMSCs to osteoblasts)”, undergraduate and postgraduate teaching (Mutagenesis and Carcinogenesis, Research Methods in Molecular Biology, Stem Cell Biology), publications in high profile scientific journals, presentations on international scientific meetings. Supervision: Inga Marijanović, PhD
2013-2014 Postdoctoral Fellow, Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, USA
Research in stem cell biology and lineage tracing of osteoprogenitor cells. Supervision: Ivo Kalajzić, M.D., PhD
2006-2012 Junior Assistant, PhD student, Faculty of Science, University of Zagreb, Croatia
Research in cancer and bone biology; Scientific project title: Effect of FADD expression during UV carcinogenesis; Project management, data analysis, undergraduate and postgraduate teaching (Mutagenesis and Carcinogenesis, Research Methods in Molecular Biology, Stem Cell Biology), publications in high profile scientific journals, presentations on international scientific meetings. Supervision: Biserka Nagy, PhD, Inga Marijanović, PhD
2011-2012 Visiting Researcher at Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, USA
Research of mesenchymal progenitor cells, evaluation of NPY as a regulator of skeletogenesis and cloning. Supervision: Ivo Kalajzić, M.D., PhD
2010 Visiting Researcher at University Hospital Centre Groningen, Department of Hematology and Department of Stem Cell Biology, The Nederlands
Single-cell multiplex PCR, data analysis. Supervision: Edo Vellenga, M.D., PhD, Hein Schepers, PhD
2004-2006 Researcher at University Hospital Centre Zagreb, Croatia
Research of mutations of N-myc and of the locus 11q23 in neuroblastoma. Supervision: Branko Malenica, M.D., PhD
ACHIEVEMENTS
2014 American Society for Bone and Mineral Research plenary poster travel award
2012 The Lawrence G. Raisz Award
2010 Coimbra Group Hospitality Scheme Fellowship
2006 Student Scholarship from Kastav City
2000-2006 Scholarship from the Croatian Ministry of Science and Technology
2000 Biology state competition, Makarska, Croatia, the 1st place
LABORATORY SKILLS
RNA and DNA extraction and electrophoresis, quantitative RT-PCR, single-cell multiplex PCR and PCR, cloning and recombinant DNA methods, paraffin and Cryomatrix embedding and histological analysis, immunocytochemistry and immunohistochemistry analysis, protein extraction, quantification, and Western blot, ELISA, EdU cell proliferation assays (in vitro and in vivo), MTT cell viability assay, the luciferase reporter assay, cell culture and differentiation in bioreactors, primary cell culture (bone marrow, muscle, tendon, cartilage, bone), organ culture (calvaria), live cell imaging, bright field and fluorescence microscopy, adenoviruses: propagation, purification by CsCl equilibrium density gradient centrifugation, quantification of viral particles and determination of plaque formation units, adenoviral transduction of primary cell culture and human tissues, mouse handling, genotyping and IP injection
COMPUTER SKILLS
Bioinformatic tools for Protein and DNA analysis, Microsoft Office, Statistical programs (GraphPrism), ImageJ.
LANGUAGES
Croatian (native), English (fluent), Italian (fair), German (fair)
PERSONAL INTERESTS
Music, travel, skiing, volleyball, diving
SCIENTIFIC ACHIEVEMENTS
PUBLICATIONS
Matic I, Antunovic M, Brkic S, Josipovic P, Caput Mihalic K, Karlak I, Ivkovic A, Marijanovic I. 2016: Expression of OCT-4 and SOX-2 in Bone Marrow-Derived Human Mesenchymal Stem Cells during Osteogenic Differentiation. OA Maced J Med Sci January 18; http://dx.doi.org/10.3889/oamjms.2016.008
Matthews BG, Torreggiani E, Roeder E, Matic I, Grcevic D, Kalajzic I. 2015: Osteogenic potential of alpha smooth muscle actin expressing muscle resident progenitor cells. Bone. Dec 22;84:69-77.
Matthews BG, Roguljic H, Franceschetti T, Roeder E, Matic I, Vidovic I, Joshi P, Kum KY, Kalajzic I. 2015: Gene-expression analysis of cementoblasts and osteoblasts. J Periodontal Res. Jul 27
Pauley P, Matthews BG, Wang L, Dyment NA, Matic I, Rowe DW, Kalajzic I. 2014: Local transplantation is an effective method for cell delivery in the osteogenesis imperfecta murine model. Int Orthop. Jan 3.
Torreggiani E, Matthews BG, Pejda S, Matic I, Horowitz MC, Grcevic D, Kalajzic I 2013: Preosteocytes/osteocytes have the potential to dedifferentiate becoming a source of osteoblasts. PLoS One. Sep 6;8(9):e75204. doi: 10.1371/journal.pone.0075204. eCollection 2013.
Matic I, Matthews BG, Kizivat T, Igwe JC, Marijanovic I, Ruohonen ST, Savontaus E, Adams DJ, Kalajzic I 2012: Bone-specific overexpression of NPY modulates osteogenesis. J Musculoskelet Neuronal Interact. Dec;12(4):209-18.
Jurela A, Repic D, Pejda S, Juric H, Vidakovic R, Matic I, Bosnjak A 2012: The effect of two different bracket types on the salivary levels of S mutans and S sobrinus in the early phase of orthodontic treatment. Angle Orthod. Jul 5.
Marijanovic I, Radnic M, Matic I, Erceg-Ivkosic I 2010: Osteogenesis imperfecta and achievements in cell and gene therapy. Acta Med Croatica Jul;64(3):191-200.
Matic I, Radnic M, Furcic I, Nagy B 2009: Biotechnology and Immortalized cells. Technologica Acta 2: 5-12.
Matic I, Radnic M, Marijanovic I, Furcic I, Nagy B 2008: Effect of UVC irradiation on p53 in FADD+/+ and FADD-/- cells. IRPA12 International Congress of the International Radiation Protection Association, Proceedings CD (TS III.2.1. 3406).
Radnic M, Kalinic L, Matic I, Ruso A, Marijanovic I, Furcic I, Nagy B 2008: Effect of FADD expression during UVC carcinogenesis. IRPA12 International Congress of the International Radiation Protection Association, Proceedings CD (TS III.2.1. 3407).
Rogina A, Ressler A, Matić I, Gallego Ferrer G, Marijanović I, Ivanković M, Ivanković H. 2017: Cellular hydrogels based on pH-responsive chitosan-hydroxyapatite system. Carbohydr Polym. Jun 15;166:173-182. doi: 10.1016/j.carbpol.2017.02.105. Epub Feb 27.
Cuti T, Antunovic M, Marijanovic I, Ivkovic A, Vukasovic A, Matic I, Pecina M, Hudetz D. 2017: Capacity of muscle derived stem cells and pericytes to promote tendon graft integration and ligamentization following anterior cruciate ligament reconstruction. Int Orthop. Jun;41(6):1189-1198. doi: 10.1007/s00264-017-3437-y. Epub Mar 15. Erratum in: Int Orthop. 2017 Jun;41(6):1287.
Matic I, Matthews BG, Wang X, Dyment NA, Worthley DL, Rowe DW, Grcevic D, Kalajzic I. 2016: Quiescent Bone Lining Cells Are a Major Source of Osteoblasts During Adulthood. Stem Cells. 2016 Aug 10. doi: 10.1002/stem.2474.
Begović L, Antunovic M, Matic I, Furcic I, Baricevic A, Vojvoda Parcina V, Peharec Štefanić P, Nagy B, Marijanovic I. 2016: Effect of UVC radiation on mouse fibroblasts deficient for FAS-associated protein with death domain. Int J Radiat Biol. 2016 Aug;92(8):475-82. doi:
10.1080/09553002.2016.1186298. Epub 2016 Jun 3.
CONFERENCE REPORTS
Annual Meeting of American Society for Bone and Mineral Research (ASBMR 2014), Houston, USA, 2014; Matic I, Matthews B, Kalajzic I “Bone lining cells are a major source of osteoblasts during bone remodeling” (Plenary Poster presentation)
2nd International Conference on Regenerative Orthopaedics and Tissue Engineering, Opatija, Croatia, 2012; Matic I, Ivkovic A, Brkic S, Josipovic P, Karlak I, Pecina M, Marijanovic I. “Osteogenic potential and stemness of mesenchymal stem cells after dexamethasone treatment” (Poster presentation)
Annual Meeting of American Society for Bone and Mineral Research (ASBMR 2011), San Diego, USA, 2011; Matic I, Igwe J, Repic D, Pejda S, Kizivat T, Adams D, Kalajzic I “Bone-specific Overexpression of NPY Modulates Osteogenic Differentiation” (Poster presentation)
20th European Association for Cancer Research conference, Oslo, Norway, 2010; Matic I, Radnic M, Marijanovic I, Caput-Mihalic K, Furcic I, Nagy B „Absence or low expression of Fas-associated protein with death domain in acute leukemia and lymphoma cell lines“ (Poster presentation)
IRPA12 International Congress of the International Radiation Protection Association, Buenos Aires, Argentina, 2008; Radnic M, Matic I, Kalinic L, Ruso A, Vojvoda V, Marijanovic I, Furcic I, Nagy B “Effect of FADD expression during UVC carcinogenesis” (Poster presentation)
IRPA12 International Congress of the International Radiation Protection Association, Buenos Aires, Argentina, 2008; Matic I, Radnic M, Marijanovic I, Furcic I, Nagy B “Effect of UVC irradiation on p53 in FADD+/+ and FADD-/- cells” (Oral and poster presentation)
20th European Association for Cancer Research conference, Lyon, France, 2008; Matic I, Furcic I, Nagy B
„Genetic instability of FADD-deficient mouse cells“ (Poster presentation)
The 4th Croatian Congress in Human Genetics with international participation, Malinska, Croatia, 2007; Matic I, Malenica B, Davidovic-Mrsic S, Franjic-Simic I, Jelasic D, Batinica D, Čavar S “Mutations of N-myc and of the locus 11q23 in neuroblastoma” (Poster presentation)